De novo sequencing is an effective mothed to acquire a novel genome without reference genome. To comprehend the fungus genome structure and protein function, the genome assembly and annotation are available. Personalbio offers two projects including fungus draft genome and approximately complete genome by using various sequencing platforms in fungus genome sequencing. The integrate strategy was provided from DNA extraction to personalized analysis by supervision of experts.
Project Workflow
Project Parameters
DNA amount* mentioned above is just for once experiment. In fact, three times DNA is recommended in library construction.
No contamination* means the DNA should be provided with the OD260/280 ratio 1.8-2.0 and OD260/230 ratio 2.0-2.2.
Simple genome* refers to the species genomes which heterozygosity less than 0.5% and repeat content less than 15%.
Complex genome* refers to the species genome which heterozygosity higher than 0.5% and repeat content higher than 15%.
