Digital RNA sequencing is based on unique molecular identifiers (UMI) technology to mark all the reverse transcribed cDNA fragments before the amplification of cDNA. It can accurately obtain the original number of cDNA and realize the precise quantification by calculating the number of UMIS connected with cDNA.
Advantages
1. Digital RNA-seq can obtain more accurate quantitative results even for low abundance expression genes and high consistency with qPCR verification results.
2. Digital RNA-seq can easily distinguish between natural duplication and amplified duplication, and accurately identify alternative splicing events.
3. Digital RNA-seq can correct the base error and precisely analysis RNA editing and SNP detection.
